Human Proteoglycan 4 (PRG4) ELISA Kit

Cat.#:

JL14598

Regulatory Status:

RUO

Alternative:

MSF; CACP; HAPO; JCAP; SZP; Megakaryocyte Stimulating factor; Articular Superficial Zone Protein; Camptodactyly; Arthropathy;Coxa Vara;Pericarditis Syndrome

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

62.5-4000pg/mL

Sensitivity:

31.54pg/mL

Specificity:

This kit recognizes Human PRG4 in samples. No significant cross-reactivity or interference between Human PRG4 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenatesand other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PRG4

Proteoglycan 4 or lubricin is a proteoglycan that is encoded by the PRG4 gene.It acts as a joint/boundary lubricant.Lubricin is present in synovial fluid and on the surface (superficial layer) of articular cartilage and therefore plays an important role in joint lubrication and synovial homeostasis. When first isolated, cartilage lubricin was called "superficial zone protein" (SZP).Due to the discovery that the 32-kDa amino terminal fragment of lubricin could stimulate in-vitro megakaryocyte growth, the gene responsible for the expression of lubricin was initially called "megakaryocyte-stimulating factor" (MSF).However, Lubricin, MSF, and SZP are now collectively known as Proteoglycan 4 (hence PRG4 for the gene nomenclature).The expression of lubricin has also been detected and the protein localized in tendon,meniscus,lung, liver, heart, bone,ligament, muscle, and skin.It is present in human plasma, where it binds to neutrophils via L-selectin.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	4000	2000	1000	500	250	125	62.5	0
OD	2.22	1.96	1.38	0.97	0.55	0.31	0.19	0.08
Corrected OD	2.44	1.88	1.3	0.88	0.47	0.23	0.11	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human PRG4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Human PRG4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98